Cytokeratin 14/17, CK14/17 Antibody

• Catalog Number: orb1294304
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Cytokeratin 14
• Target: Cytokeratin 14/17, CK14/17
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit-IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Immunogen: Synthetic peptide / encompassing a sequence within the center region
• Tested applications: IF, IHC, WB
• Dilution range: Western Blot 1:1000–1:2000 Immunofluorescence 1:100–1:400 Immunohistochemistry (Paraffin) 1:100–1:300
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Research Area: Cancer Biology
• Note: For research use only

All lanes: Anti-Cytokeratin 14/17 antibody - at 1/2000 dilution, Lysates/proteins at 45 µg per lane. This blot was produced using a 10% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked an hour before being incubated with orb1294304 overnight at 4°C.

Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294304 anti-Cytokeratin 14/17 antibody (red) at 1:150 and 4°C. DAPI (blue) was used as the nuclear counter stain.

Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294304 anti-Cytokeratin 14/17 antibody (red) at 1:150 and 4°C. DAPI (blue) was used as the nuclear counter stain.

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling Cytokeratin 14/17 with orb1294304 at 1/100.

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling Cytokeratin 14/17 with orb1294304 at 1/100.

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling Cytokeratin 14/17 with orb1294304 at 1/100.

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling Cytokeratin 14/17 with orb1294304 at 1/100.