Cyclooxygenase 2 Rabbit Polyclonal Antibody

SKU: orb182638

Description

Cyclooxygenase 2 Rabbit Polyclonal Antibody

Research Area

Cancer Research, Cardiovascular Research, Cholesterol Metabolism, Hormone Biosynthesis, Inflammation, Macrophage, Metabolism, Prostaglandin, Tumor Biomarkers

Images & Validation

• Tested Applications: FC, ICC
• Dilution Range: ICC/IF=1:50-200, Flow-Cyt=1:100-200
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Equine, Gallus, Mouse, Porcine, Rabbit, Rat, Sheep

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Cyclooxygenase 2 (301-400/604aa)
• Target: Ptgs2
• Molecular Weight: 65 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

COX-2; COX2; GRIPGHS; PGG/HS; PGHS-2; PHS-2; hCox-2; PES-2; PHS II; Pghs2; TIS10; PGH2_HUMAN; PTGS2; Cyclooxygenase-2 (COX-2); Prostaglandin H2 synthase 2 (PGH synthase 2 | PGHS-2); Prostaglandin-endoperoxide synthase 2; 1.14.99.1; PGH2_MOUSE; Glucocorticoid-regulated inflammatory cyclooxygenase; Macrophage activation-associated marker protein P71/73; TIS10 protein; Pghs-b; PGH2_RAT;

Sample: Lane 1: Recombinant human COX2 protein, Primary: Anti-Cyclooxygenase 2 (orb182638) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 65 kD, Observed band size: 40 kD.

Tissue/Cell: human myometrium tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-Cyclooxygenase-2 Polyclonal Antibody, Unconjugated (orb182638) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Tissue/Cell: human prostate tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-COX-2 Polyclonal Antibody, Unconjugated (orb182638) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Tissue/Cell: mouse brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-COX-2 Polyclonal Antibody, Unconjugated (orb182638) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

Ptgs2

UniProt

P35354