Collagen III Rabbit Polyclonal Antibody

SKU: orb10437

Description

Collagen III Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: ICC, IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ICC/IF=1:50-200
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Gallus, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Collagen alpha 1(III) chain (1301-1400/1466aa)
• Target: COL3A1
• Molecular Weight: 117 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

COL 3A1; COL3A1; Collagen alpha 1(III) chain; Collagen III alpha 1 chain precursor; Collagen III alpha 1 polypeptide; Collagen type III alpha 1(Ehlers Danlos syndrome type IV autosomal dominant); Collagen type III alpha 1; Collagen type III alpha; EDS4A; Ehlers Danlos syndrome type IV, autosomal dominant; Fetal collagen; Type III collagen; CO3A1_HUMAN; Collagen alpha-1(III) chain; Type III collagen; type III preprocollagen alpha 1 chain.

Sample: Lane 1: Human HeLa cell lysates, Lane 2: Human MCF-7 cell lysates, Primary: Anti-Collagen III (orb10437) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 117 kDa, Observed band size: 130 kDa.

Sample: MCF-7 (Human) Cell Lysate at 30 ug, Hela (Human) Cell Lysate at 30 ug, Primary: Anti-Collagen III (orb10437) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 120/170 kD, Observed band size: 170 kD.

Sample: Raji (human) cell Lysate at 40 ug, Primary: Anti-Collagen III (orb10437) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 117 kD, Observed band size: 140 kD.

Quick Database Links

Gene Symbol

COL3A1

UniProt

P02461

References

Youqin Jiang # 1, Yao Su # 1, Chen Li 1, Weiwei Jiang 1, Yang Wei 1, Guanglei Chang 2, Ya Liu 1, Honghong He Geniposide alleviates post-myocardial infarction-induced pyroptosis by modulating the thioredoxin-interacting protein/NLRP3 signaling pathway Cytojournal ., (2025)

PubMed: 39916997

Applications

IHC

Reactivity

Mouse