Collagen I/COL1A2 Rabbit pAb Discontinued

SKU: orb373292

This product can be custom made

Description

Rabbit polyclonal antibody to CO1A2

Research Area

Epigenetics

Images & Validation

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Item 1 of 4

Tested Applications	ELISA, IF, IHC-P, WB
Dilution Range	WB: 1:500-2000
Reactivity	Human, Mouse, Rat

Key Properties

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Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	A synthetic peptide corresponding to a sequence within amino acids 501-600 of human Collagen I/COL1A2 (NP_000080.2).
Molecular Weight	165kDa
Purification	Affinity purification
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	PBS with 0.05% proclin300,50% glycerol,pH7.3.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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anti-CB11 antibody, anti-COL11A3 antibody, anti-Dmm antibody, anti-Col2a-1 antibody, anti-Del1 antibody, anti-Lpk antibody, anti-Col2a antibody, anti-M100856 antibody, anti-Rgsc856 antibody, anti-ANFH antibody, anti-Col2 antibody, anti-BOS_5148 antibody, anti-SEDC antibody, anti-CG2A1A antibody, anti-COLLII antibody, anti-AOM antibody, anti-Collagen II antibody, anti-collagen II antibody

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Immunofluorescence analysis of A-431 cells using Collagen I/COL1A2 Rabbit pAb (orb373292) at dilution of 1:25. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH/3T3 cells using Collagen I/COL1A2 Rabbit pAb (orb373292) at dilution of 1:25. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunohistochemistry analysis of paraffin-embedded Human placenta using Collagen I/COL1A2 Rabbit pAb (orb373292) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Western blot analysis of various lysates, using Collagen I/COL1A2 Rabbit pAb (orb373292) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Negative control (NC):JurkatExposure time: 30s.