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Description
Research Area
Images & Validation
−| Tested Applications | FC, WB |
|---|---|
| Dilution Range | WB - 1:1000, FC - 1:10-50 |
| Reactivity | Mouse |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Clone No. | RB18892 |
| Immunogen | This COL4A2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 156-186 amino acids from the N-terminal region of human COL4A2. Antigen Region: 156-186 aa. |
| Target | COL4A2 (HGNC:2203) |
| Molecular Weight | 167553 Da |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
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Anti-COL4A2 Antibody (N-term) at 1:1000 dilution + Mouse kidney whole tissue lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 168 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

COL4A2 Antibody (N-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Quick Database Links
UniProt Details
−NCBI Reference Sequences
−| Protein | NP_001837.2 |
|---|
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Protocol Information
COL4A2 Antibody (N-term) (orb1938185)
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