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| Catalog Number | orb700358 |
|---|---|
| Category | Antibodies |
| Description | Gallus polyclonal antibody against Gallus anti-Rabbit IgG (H&L) conjugated to HRP |
| Clonality | Polyclonal |
| Species/Host | Gallus |
| Conjugation | HRP |
| Reactivity | Rabbit |
| Form/Appearance | Lyophilized |
| Purity | Immunogen affinity purified chicken IgY. |
| Purification | HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free0.1 % (v/v) of Kathon CG is used as preservative. Use of sodium azide will inhibit enzyme activity of horseradish peroxidase |
| Immunogen | Purified Rabbit IgG |
| Tested applications | ELISA, IHC, WB |
| Dilution range | The optimal working dilution should be determined by the investigator |
| Application notes | This antibody reacts with the heavy chains on rabbit IgG and with the light chains on all rabbit immunoglobulins based on immunoelectrophoresis.No reactivity is observed to non-immunoglobulin rabbit serum proteins and human and mouse serum and IgG on immunoelectrophoresis.This antibody was absorbed against solid phase human and mouse serum and IgG. |
| Storage | Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming. |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Rabbit | |
Donkey | |
Polyclonal | |
HRP |
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