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Catalog Number | orb700356 |
---|---|
Category | Antibodies |
Description | Gallus polyclonal antibody against Gallus anti-Rabbit IgG (H&L) conjugated to AP |
Clonality | Polyclonal |
Species/Host | Gallus |
Conjugation | ALP |
Reactivity | Rabbit |
Form/Appearance | Liquid |
Purity | Immunogen affinity purified chicken IgY. |
Purification | ALP conjugate is supplied in 30 mM Triethanolamine, pH 7,2, 5 mM Magnesium Chloride, 0,1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) of sodium azide is added as preservative |
Immunogen | Purified Rabbit IgG |
Tested applications | ELISA, ICC, IHC, WB |
Dilution range | 1 : 500-1 :2000 (ELISA), 1 : 50-1 : 5000 (ICC), 1 : 20 -1 : 2000 (IHC), 1 : 500-1 :2000 (WB) |
Application notes | This antibody reacts with the heavy chains on rabbit IgG and the light chains on all rabbit immunoglobulins based on immunoelectrophoresis.No reactivity is observed to non-immunoglobulin rabbit serum proteins or to human or mouse serum or IgG based on immunoelectrophoresis. |
Storage | Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming. |
Note | For research use only |
Rabbit | |
Donkey | |
Polyclonal | |
ALP |