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Catalog Number | orb1928097 |
---|---|
Category | Antibodies |
Description | Purified Rabbit Polyclonal Antibody (Pab) |
Target | This CFLAR antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 145-174 amino acids from the Central region of human CFLAR. |
Clonality | Polyclonal |
Species/Host | Rabbit |
Isotype | Rabbit IgG |
Conjugation | Unconjugated |
Reactivity | Human |
Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
UniProt ID | O15519 |
MW | 55344 Da |
Tested applications | FC, IHC-P, WB |
Dilution range | WB: 1:1000, IHC-P: 1:50~100, FC: 1:10~50 |
Antibody Type | Primary Antibody |
Clone Number | RB18044 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
Alternative names | CASP8 and FADD-like apoptosis regulator, Caspase h Read more... |
Note | For research use only |
NCBI | NP_001120655.1, NP_001120656.1, NP_001189447.1, NP_001189444.1, NP_001189446.1, NP_003870.4, NP_001189448.1, NP_001189445.1 |
Western blot analysis of CFLAR Antibody (Center) in HepG2 cell line lysates (35 ug/lane). CFLAR (arrow) was detected using the purified Pab.
CFLAR Antibody (Center) flow cytometric analysis of k562 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Formalin-fixed and paraffin-embedded human skeletal muscle reacted with CFLAR Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.