Cdk1/2/3 (phospho Thr14) rabbit pAb

• Catalog Number: orb764160
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Cdk1/2/3 (phospho-Thr14)
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Monkey, Mouse, Rat
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Immunogen: The antiserum was produced against synthesized peptide derived from human CDK1/CDC2 around the phosphorylation site of Thr14. AA range:1-50
• MW: 34
• Tested applications: ELISA, IF, WB
• Dilution range: Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Anti-CDK1 antibody, anti-CDC2 antibody, anti-CDC28
• Note: For research use only

Western Blot analysis of various cells using Phospho-Cdk1/2/3 (T14) Polyclonal Antibody diluted at 1:2000.

Western Blot analysis of COS7 cells using Phospho-Cdk1/2/3 (T14) Polyclonal Antibody diluted at 1:2000.

Immunofluorescence analysis of COS7 cells, using CDK1/CDC2 (Phospho-Thr14) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of lysates from HepG2 cells treated with Forskolin 40nM 30', using CDK1/CDC2 (Phospho-Thr14) Antibody. The lane on the right is blocked with the phospho peptide.