CD31 Rabbit Polyclonal Antibody

SKU: orb1294369

Description

Rabbit polyclonal antibody to CD31

Research Area

Cardiovascular Research

Images & Validation

• Tested Applications: IHC, WB
• Dilution Range: Western Blot 1:500-1:1000, Immunohistochemistry (Frozen) 1:100-1:200, Immunohistochemistry (Paraffin) 1:100-1:200
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit-IgG
• Immunogen: Synthetic peptide / Extracellular domain corresponding to Human CD31
• Target: CD31 / PECAM1 (Extracellular)
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 100mM Tris Glycine, 1% rAlbumin, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
• Disclaimer: For research use only

Anti-CD31/PECAM1 antibody at 1/500 dilution, Lysates at 30 µg per lane, This blot was produced using a 7.5% SDS-PAGE. Nitrocellulose membrane was then blocked with 3% BSA for an hour before being incubated with orb1294369 overnight at 4°C.

Anti-CD31/PECAM1 antibody at 1/500 dilution, Lysates at 30 µg per lane, This blot was produced using a 7.5% SDS-PAGE. Nitrocellulose membrane was then blocked with 3% BSA for an hour before being incubated with orb1294369 overnight at 4°C.

Anti-CD31/PECAM1 antibody at 1/500 dilution, Lysates at 30 µg per lane, This blot was produced using a 7.5% SDS-PAGE. Nitrocellulose membrane was then blocked with 3% BSA for an hour before being incubated with orb1294369 overnight at 4°C.

Immunohistochemical analysis of paraffin embedded Human liver cancer tissue labeling CD31 with orb1294369 at 1/100.

Immunohistochemical of frozen sections. Sample: liver. Green: CD31 (orb1294369): 1-100.

Quick Database Links

Gene Symbol

CD31 / PECAM1 (Extracellular)