CD31 Antibody (C-term)

SKU: orb1929230

Description

Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: FC, WB
• Dilution Range: WB - 1:2000, FC - 1:25
• Reactivity: Human

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB17999
• Immunogen: This CD31 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 690-718 amino acids from the C-terminal region of human CD31. Antigen Region: 690-718 aa.
• Target: PECAM1
• Molecular Weight: 82522 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Platelet endothelial cell adhesion molecule, PECAM-1, EndoCAM, GPIIA', PECA1, CD31, PECAM1

Western blot analysis of CD31 antibody (C-term) in HL60 cell line lysates (35 ug/lane). CD31 (arrow) was detected using the purified Pab.

All lanes: Anti-CD31 Antibody (C-term) at 1:2000 dilution. Lane 1: Jurkat whole cell lysate. Lane 2: human lung lysate. Lane 3: THP-1 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 83 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Overlay histogram showing THP-1 cells stained (green line). The cells were fixed with 2% paraformaldehyde (10 min). The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37°C. Isotype control antibody (blue line) was rabbit IgG (1 μg/1x10^6 cells) used under the same conditions. Acquisition of > 10000 events was performed.

Quick Database Links

Gene Symbol

PECAM1

UniProt

P16284

RefSeq (Protein):NP_000433.4