CD28 Rabbit Polyclonal Antibody

SKU: orb10295

Description

CD28 Rabbit Polyclonal Antibody

Research Area

CD Markers, Cell Biology, Immunology, Innate Immunity, Natural Killer Cell Lineage, Stem Cell Research

Images & Validation

• Tested Applications: FC, IF, IHC-Fr, IHC-P
• Dilution Range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1μg/Test
• Reactivity: Mouse, Rat
• Predicted Reactivity: Bovine, Canine, Equine, Gallus, Human, Porcine

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from mouse CD28 (1-120/218aa)
• Target: CD28
• Molecular Weight: 45 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CD 28; CD28 antigen; CD28 molecule; MGC138290; T cell antigen CD28; T cell specific surface glycoprotein; T cell specific surface glycoprotein CD28; Tp44; CD28_HUMAN; CD28_MOUSE.

Paraformaldehyde-fixed, paraffin embedded (rat stomach tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (CD28) Polyclonal Antibody, Unconjugated (orb10295) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Positive control: (mo) Splenocytes (2% Paraformaldehyde-fixed), Isotype Control Antibody, Antibody: Rabbit IgG, Dilution: 1 µg in 100 µl 1 X PBS containing 0.5% BSA, Secondary Antibody, Antibody: Goat anti-rabbit IgG-FITC, Dilution: 1:200 in 1 X PBS containing 0.5% BSA, Primary Antibody, catalog number: orb10295, Dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA.

Tissue/cell: mouse lung tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-CD28 Polyclonal Antibody, Unconjugated (orb10295) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

CD28

UniProt

P31041