CD274 antibody

SKU: orb10162

Description

CD274 antibody

Images & Validation

• Tested Applications: ELISA, IHC-P, WB
• Dilution range: IHC-P: 1:50-300 (based on 0.5mg/ml), WB: 1:100-500
• Reactivity: Human, Mouse, Rat

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human CD274. Please contact us for the exact immunogen sequence. The peptide is available as orb374702.
• Target: CD274
• Molecular Weight: 33 kDa
• Purity: Polyclonal antibodies are purified by peptide affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: 10 mM PBS, 0.02% sodium azide
• Concentration: - 100 μg (in 200 μl): 0.5 mg/ml- 200 μg (in 400 μl): 0.5 mg/ml
• Disclaimer: For research use only

Alternative Names

Anti-CD274 antibody, anti-Programmed cell death 1 ligand 1 antibody, anti-Programmed death ligand 1 antibody, anti-B7 homolog 1 antibody, anti-B7-H1 antibody.

Western blot analysis of mouse heart (lane 1), rat muscle (lane 2), rat lung (lane 3), rat thymus (lane 4), rat lymph node (lane 5) using CD274 antibody (2.5 ug/ml)

Immunohistochemical staining of paraffin embedded rat thymus tissue using anti-CD274 (2.5 ug/ml)

IHC-P staining of rat thymus tissue using anti-CD274 (2.5 ug/ml)

IHC-P staining of rat thymus tissue using anti-CD274 (2.5 ug/ml)

IHC-P image of mouse spleen tissue using anti-CD274 (2.5 ug/ml)

IHC-P image of rat heart tissue using CD274 antibody (2.5 ug/ml)

Immunohistochemical staining of paraffin embedded rat thymus tissue using anti-CD274 (2.5 ug/ml)

Immunohistochemical staining of paraffin embedded rat heart tissue using CD274 antibody (2.5 ug/ml)

Immunohistochemical staining of rat heart tissue using CD274 antibody (2.5 ug/ml)

WB analysis of mouse heart (lane 1), rat muscle (lane 2), rat lung (lane 3), rat thymus (lane 4), rat lymph node (lane 5) using CD274 antibody (2.5 ug/ml)

Western blot analysis of mouse heart, rat lung, rat spleen, A549, human breast cancer, human lung cancer (Lane 1 to Lane 6) using CD274 antibody (1 ug/ml)

Western blot analysis of mouse heart, rat lung, rat spleen, A549, human breast cancer, human lung cancer (Lane 1 to Lane 6) using CD274 antibody (1 ug/ml)

Immunohistochemical staining of human breast cancer tissue using CD274 antibody (2.5ug/ml)

Immunohistochemical staining of paraffin embedded human breast cancer tissue using CD274 antibody (2.5ug/ml)

Quick Database Links

Gene Symbol

CD274

UniProt

Q9EP73

NCBI Gene ID

29126

References

Hubert, Pascale et al. Extracellular HMGB1 blockade inhibits tumor growth through profoundly remodeling immune microenvironment and enhances checkpoint inhibitor-based immunotherapy J Immunother Cancer, 9, (2021)

PubMed: 33712445