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CD22 Antibody
SKU: orb153503
Featured
Description
Images & Validation
−Item 1 of 7
| Tested Applications | FC, ICC, IHC, WB |
|---|---|
| Dilution range | WB: 1/500 - 1/2000; IHC: 1/200 - 1/1000; ICC: 1/50 - 1/200; FCM: 1/200 - 1/400; ELISA: 1/10000 |
| Reactivity | Human |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | Mouse IgG1 |
| Clone No. | 2H1C4 |
| Immunogen | Purified recombinant fragment of human CD22 (AA: 621-725) expressed in E. Coli. |
| Target | CD22 |
| Molecular Weight | 95.3kDa |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Purified antibody in PBS with 0.05% sodium azide |
| Concentration | Approximately 1mg/ml (varies from batch to batch). Please inquire for precise concentration. |
| Disclaimer | For research use only |
Alternative Names
−SIGLEC2; SIGLEC-2
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Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].
Western blot analysis of HEK293 (Lane 1) HEK293 (Lane 2) cell lysate using CD22 antibody
Western blot analysis of L1210 (Lane 1), Hela (Lane 2), HEK293 (Lane 3), Jurkat (Lane 4), OCM-1 (Lane 5), A432 (Lane 6) NIH/3T3 (Lane 7) cell lysate using CD22 antibody
Line graph illustrates about the Ag-Ab reactions using different concentrations of antigen and serial dilutions of CD22 antibody
Flow cytometric analysis of Hela cells lysates using CD22 antibody
Immunohistochemical analysis of formalin-fixed and paraffin-embedded cervical cancer tissues using CD22 antibody
Immunohistochemical analysis of formalin-fixed and paraffin-embedded cerebellum tissues using CD22 antibody
Western blot analysis of human CD22 using CD22 antibody
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Datasheet
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
FC
Flow Cytometry
ICC
Immunocytochemistry
CD22 Antibody (orb153503)
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