CD22 Antibody

• Catalog Number: orb689339
• Category: Antibodies
• Description: Anti-CD22 antibody(DM13), Rabbit mAb
• Target: CD22
• Clonality: Monoclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Liquid
• Purification: Purified from cell culture supernatant by affinity chromatography
• UniProt ID: P20273
• Tested applications: ELISA, FC
• Dilution range: Flow Cyt 1:100
• Application notes: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.
• Antibody Type: Recombinant Antibody
• Clone Number: DM13
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: SIGLEC-2, SIGLEC2
• Note: For research use only

Expi 293 cell line transfected with irrelevant protein (left) and human CD22 (right) were surface stained with Rabbit anti- CD22 monoclonal antibody 1 μg/ml (clone: DM13) followed by Alexa 488-conjugated anti-rabbit IgG secondary antibody.

Flow cytometry data of serially titrated Rabbit anti-CD22 monoclonal antibody (clone: DM13) on Raji cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.

Affinity ranking of different Rabbit anti-CD22 mAb clones by titration of different concentration onto Raji cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.

Phylogenetic analysis of amino acid sequence of different Rabbit Anti-CD22 mAb clones. A) Heavy chain and B) Light chain.

Flow cytometry analysis of antigen binding of rabbit anti-human CD22 mAb (orb689339). (A) orb689339 does not bind to Jurkat cells that do not express CD22. (B) A clear peak shift of orb689339 was seen compared to the control when incubated with CD22-expressing Raji cells, indicating strong binding of orb689339 to CD22. Antibodies were incubated at 10 μg/mL.