Caspase-8 Antibody

• Catalog Number: orb1239264
• Category: Antibodies
• Description: Caspase-8 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, ICC, IF, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Caspase-8 antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human Caspase-8 isoform A.The immunogen is located within the last 50 amino acids of Caspase-8.
• Antibody Type: Primary Antibody
• Concentration: 1 mg/mL
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 55 kDa
• Target: CASP8
• UniProt ID: Q14790
• NCBI: NP_001219
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Caspase-8 Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: Caspase-8 Antibody: CAP4, MACH, MCH5, FLICE, ALPS2
• Note: For research use only
• Application notes: Caspase-8 antibody can be used for the detection of caspase-8 by Western blot at 0.5 to 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 2 μg/mL and immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in human, mouse, and rat samples; Immunohistochemistry in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
• Expiration Date: 12 months from date of receipt.

Immunocytochemistry of caspase-8 in Jurkat cells with caspase-8 antibody at 2 µg/mL.

Immunofluorescence of Caspase-8 in human spleen tissue with Caspase-8 antibody at 20 µg/mL. Red: Caspase-8 Antibody (orb1239264). Blue: DAPI staining.

Immunofluorescence of Caspase-8 in Jurkat cells with Caspase-8 antibody at 20 µg/mL.

Immunohistochemistry of Caspase-8 in human spleen tissue with Caspase-8 antibody at 5 µg/mL.

WB Validation in Human K562 Cells. Loading: 15 µg of K562 cell lysate Antibodies: Casp8 orb1239264, 1 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.

WB Validation in Mouse 3T3/NIH Cells. Loading: 15 µg of 3T3/NIH cell lysate Antibodies: Casp8 orb1239264, 1 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.

WB Validation in Rat YB2/0 Cells. Loading: 15 µg of YB2/0 cell lysate Antibodies: Casp8 orb1239264, 1 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.