c-KIT Antibody (N-term)

SKU: orb1939483

Description

Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Signal Transduction

Images & Validation

• Tested Applications: FC, IHC-P, WB
• Dilution Range: WB - 1:1000
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: BTD3770
• Immunogen: This c-KIT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 9-40 amino acids from the N-terminal region of human c-KIT. Antigen Region: 9-40 aa.
• Target: KIT
• Molecular Weight: 109865 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Mast/stem cell growth factor receptor Kit, SCFR, Piebald trait protein, PBT, Proto-oncogene c-Kit, Tyrosine-protein kinase Kit, p145 c-kit, v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, CD117, KIT, SCFR

Anti-c-KIT Antibody (N-term) at 1:1000 dilution+ Mouse cerebellum tissue lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 110 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Flow cytometric analysis of hela cells using KIT Antibody (N-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Purified rabbit anti-KIT Pab was used in immunohistochemistry on Breast Carcinoma.

Quick Database Links

Gene Symbol

KIT

UniProt

P10721

RefSeq (Protein):NP_001087241.1, NP_000213.1