c-KIT Antibody (C-term)

• Catalog Number: orb1939482
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Target: This c-KIT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 912-941 amino acids of human c-KIT.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Predicted Reactivity: Porcine
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• UniProt ID: P10721
• MW: 109865 Da
• Tested applications: IHC-P, WB
• Dilution range: WB: 1:1000, WB: 1:2000, IHC-P: 1:100
• Antibody Type: Primary Antibody
• Clone Number: RB1559-RB1560
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Mast/stem cell growth factor receptor Kit, SCFR, P
• Note: For research use only
• NCBI: NP_000213.1, NP_001087241.1

All lanes: Anti-c-KIT Antibody (C-term) at 1:1000 dilution. Lane 1: K562 whole cell lysate. Lane 2: TT whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 110 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-c-KIT Antibody (C-term) at 1:2000 dilution. Lane 1: Human lung lysate. Lane 2: Mouse testis lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 110 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Staining c-KIT in human breast carcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Samples were incubated with primary antibody (1/100) for 1 hours at room temperature. A undiluted biotinylated goat polyvalent antibody was used as the secondary Antibody.