BMP15 Antibody

SKU: orb1925485

Description

Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Cardiovascular Research, Immunology & Inflammation, Signal Transduction, Stem Cell & Developmental Biology

Images & Validation

• Tested Applications: IF, WB
• Dilution Range: IF - 1:25, WB - 1:4000
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: BTD78513
• Immunogen: This BMP15 antibody is generated from a rabbit immunized with a recombinant protein of human BMP15.
• Target: BMP15
• Molecular Weight: 45055 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Bone morphogenetic protein 15, BMP-15, Growth/differentiation factor 9B, GDF-9B, BMP15, GDF9B

Anti-BMP15 Antibody at 1:4000 dilution + Human testis lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 45 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized PC-3 cells labeling BMP15 at 1/25 dilution, followed by Dylight 488-conjugated goat anti-Rabbit IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing Cytoplasm and Weak Nucleus staining on PC-3 cell line. The nuclear counter stain is DAPI (blue).

Quick Database Links

Gene Symbol

BMP15

UniProt

O95972