Biotin-11-dCTP

SKU: orb64039

Description

Biotin-11-dCTP is enzymatically incorporated into DNA/cDNA as sub-stitute for its natural counterpart dCTP. The resulting Biotin-labeled DNA/cDNA probes are subsequently detected using streptavidin conjugated with horseradish peroxidase (HRP), alkaline phosphatase (AP), a fluorescent dye or agarose/magnetic beads. Optimal sub-strate properties and thus labeling efficiency as well as an efficient detection of the Biotin moiety is ensured by a 11-atom linker attached to the C5 position of cytidine. Recommended Biotin-11-dCTP/dCTP ratio for PCR and Nick Translation: 50% Biotin-11-dCTP/50% dCTP.

Images & Validation

• Application Notes:
  FISH; SNP-analysis; Nick-translation; TUNEL. ; Spectroscopic Propertie: λmax 294 nm, ε 9.3 L mmol-1 cm-1 (Tris-HCl, pH 7.5).

Key Properties

• CAS Number: 136632-30-9
• MW: Theoretical MW: 859.67 g/mol (free acid); Detected MW: 859.18 g/mol (free acid)
• Purity: ≥ 95% (HPLC)
• Formula: C₂₈H₄₄N₇O₁₆P₃S
• SMILES: N(CC#Cc1c(nc(=O)n([C@H]2C[C@@H]([C@H](O2)COP(=O)(OP(=O)(OP(=O)(O)O)O)O)O)c1)N)C(=O)CCCCCNC(=O)CCCC[C@H]1[C@H]2NC(=O)N[C@H]2CS1

Storage & Handling

• Storage: store at -20 °C
• Disclaimer: For research use only