Biotin-14-dCTP

SKU: orb533284

Description

Biotin-14-dCTP is enzymatically incorporated into DNA/cDNA as sub-stitute for its natural counterpart dCTP. The resulting Biotin-labeled DNA/cDNA probes are subsequently detected using streptavidin conjugated with horseradish peroxidase (HRP), alkaline phosphatase (AP), a fluorescent dye or agarose/magnetic beads. Optimal sub-strate properties for Nick Translation are ensured by a 14-atom linker attached to the N4 position of cytosine. For PCR incorporation experiments e.g. with Taqpolymerase Biotin-11-dCTP or Biotin-16-dCTP are recommended whose Biotin moiety is attached to C5 position of cytidine via a 11-atom or 16-atom linker, respectively. Recommended Biotin-14-dCTP/dCTP ratio for Nick Translation: 50% Biotin-14-dCTP/50% dCTP.

Images & Validation

• Application Notes:
  Incorporation into DNA/cDNA by: Nick Translation with DNAse I/DNA Polymerase I & in-house data; Primer Extension with Klenow fragment. ; Spectroscopic Propertie: λmax 272 nm, ε 13.3 L mmol-1 cm-1 (Tris-HCl, pH 7.5).

Key Properties

• MW: Theoretical MW: 905.78 g/mol (free acid); Detected MW: 905.26 g/mol (free acid)
• Purity: ≥ 95% (HPLC)
• Formula: C₃₁H₅₄N₇O₁₆P₃S
• SMILES: OP(=O)(O)OP(=O)(O)OP(=O)(O)OC[C@H]1O[C@@H](n2ccc(nc2=O)NCCCCCCNC(=O)CCCCCNC(=O)CCCC[C@@H]2SC[C@@H]3NC(=O)N[C@H]23)C[C@@H]1O

Storage & Handling

• Storage: store at -20 °C
• Disclaimer: For research use only