Biotin-11-dATP

SKU: orb1734115

Description

Biotin-11-dATP is enzymatically incorporated into DNA/cDNA as sub-stitute for its natural counterpart dATP. The resulting Biotin-labeled DNA/cDNA probes are subsequently detected using streptavidin conjugated with horseradish peroxidase (HRP), alkaline phosphatase (AP), a fluorescent dye or agarose/magnetic beads. Optimal sub-strate properties and thus labeling efficiency as well as an efficient detection of the Biotin moiety is ensured by a 11-atom linker attached to the 7-Deaza position of adenine.

Images & Validation

• Application Notes:
  Incorporation into DNA/cDNA by: PCR with Taqpolymerase & in-house data; Nick Translation with DNAse I/DNA Polymerase I; Primer Extension with Klenow exo-; Reverse Transcription with MMLV Reverse Transcriptase. Spectroscopic Propertie: λmax 280 nm, ε 12.7 L mmol-1 cm-1 (Tris-HCl, pH 7.5).

Key Properties

• MW: Theoretical MW: 882.71 g/mol (free acid); Detected MW: 882.19 g/mol (free acid)
• Purity: ≥ 95% (HPLC)
• Formula: C₃₀H₄₅N₈O₁₅P₃S
• SMILES: N1C(=O)N[C@@H]2[C@H]1[C@H](CCCCC(=O)NCCCCCC(=O)NCC#Cc1cn(c3ncnc(N)c13)[C@@H]1O[C@H](COP(=O)(O)OP(=O)(O)OP(=O)(O)O)[C@@H](O)C1)SC2

Storage & Handling

• Storage: store at -20 °C. Short term exposure (up to 1 week cumulative) to ambient temperature possible
• Disclaimer: For research use only