Biotin-14-dATP

• Catalog Number: orb1733817
• Category: Tools
• Description: Biotin-14-dATP is enzymatically incorporated into DNA/cDNA as sub-stitute for its natural counterpart dATP. The resulting Biotin-labeled DNA/cDNA probes are subsequently detected using streptavidin conjugated with horseradish peroxidase (HRP), alkaline phosphatase (AP), a fluorescent dye or agarose/magnetic beads. Optimal sub-strate properties for Nick Translation are ensured by a 14-atom linker attached to the N6 position of adenine. For PCR incorporation experiments e.g. with Taqpolymerase Biotin-11-dATP is recommended whose Biotin moiety is attached to the N7-Deaza position of adenine via a 11-atom linker. Recommended Biotin-14-dATP/dATP ratio for Nick Translation: 50% Biotin-14-dATP/50% dATP.
• Form/Appearance: filtered solution (30 kDa) in 10 mM Tris-HCl; Color: colorless to slightly yellow; pH: 7.5 ± 0.5
• Concentration: 1.0 mM-1.1 mM
• Purity: ≥ 95% (HPLC)
• MW: Theoretical MW: 929.81 g/mol (free acid); Detected MW: 929.27 g/mol (free acid)
• Application notes: Incorporation into DNA/cDNA by: Nick Translation with DNAse I/DNA Polymerase I & in-house data; Primer Extension with Klenow fragment. ; Spectroscopic Propertie: λmax 266 nm, ε 16.2 L mmol-1 cm-1 (Tris-HCl, pH 7.5).
• Formula: C₃₂H₅₄N₉O₁₅P₃S
• SMILES: OP(=O)(O)OP(=O)(O)OP(=O)(O)OC[C@H]1O[C@@H](n2cnc3c(NCCCCCCNC(=O)CCCCCNC(=O)CCCC[C@@H]4SC[C@@H]5NC(=O)N[C@H]45)ncnc23)C[C@@H]1O
• Storage: store at -20 °C. Short term exposure (up to 1 week cumulative) to ambient temperature possible
• Note: For research use only
• Expiration Date: 12 months from date of receipt.