Bid Antibody (BH3 Domain Specific)

• Catalog Number: orb1936953
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB0957, RB0958
• Tested applications: IHC-P, WB
• Reactivity: Human, Mouse
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:2000, WB: 1:1000, WB: 1:1000, WB: 1:1000, WB: 1:1000, IHC-P: 1:50~100, IHC-P: 1:50~100
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Conjugation: Unconjugated
• MW: 21995 Da
• Target: This Bid antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 68-103 amino acids from human Bid.
• UniProt ID: P55957
• NCBI: NP_001231501.1, NP_932071.1, NP_001231498.1, NP_001187.1, NP_001231496.1, NP_001231499.1, NP_932070.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: BH3-interacting domain death agonist, p22 BID, BID
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Bid (arrow) using rabbit polyclonal Bid Antibody (BH3). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the Bid gene.

Anti-Bid Antibody (BH3 Domain Specific) at 1:2000 dilution + Jurkat whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 22 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with Bid BH3 Domain Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

The anti-Bid BH3 domain Pab is used in Western blot to detect Bid BH3 in HL-60 cell lysate.

Western blot analysis of anti-hBid-BH3 Pab in mouse lung tissue lysates (35 ug/lane). hBid-BH3 (arrow) was detected using the purified Pab.

Western blot analysis of Bid (arrow) using rabbit polyclonal Bid Antibody (BH3). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the Bid gene.