BACE Antibody (S498)

SKU: orb1928903

Description

Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Cell Biology

Images & Validation

• Tested Applications: WB
• Dilution Range: WB - 1:1000
• Reactivity: Human, Mouse
• Predicted Reactivity: Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: This BACE antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 476-501 amino acids from human BACE. Antigen Region: 476-501 aa.
• Target: BACE1 (HGNC:933)
• Molecular Weight: 55764 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Beta-secretase 1, Aspartyl protease 2, ASP2, Asp 2, Beta-site amyloid precursor protein cleaving enzyme 1, Beta-site APP cleaving enzyme 1, Memapsin-2, Membrane-associated aspartic protease 2, BACE1, BACE, KIAA1149

Western blot analysis of anti-BACE Antibody (S498) in mouse cerebellum tissue lysates (35 ug/lane). BACE (arrow) was detected using the purified Pab.

All lanes: Anti-Phospho-BACE (S498) Antibody, ctrl at 1:1000 dilution. Lane 1: SH-SY5Y whole cell lysate. Lane 2: U-251 MG whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 56 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

Gene Symbol

BACE1 (HGNC:933)

UniProt

P56817

RefSeq (Protein):NP_620429.1, NP_036236.1, NP_001193978.1, NP_620427.1, NP_620428.1