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B-Myb (phospho Ser577) rabbit pAb

SKU: orb769177
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Description

Rabbit polyclonal antibody to B-Myb (phospho-Ser577)

Images & Validation

Tested ApplicationsELISA, IF, WB
Dilution rangeWestern Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.
ReactivityHuman, Mouse, Rat

Key Properties

ClonalityPolyclonal
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from human B-Myb around the phosphorylation site of Ser577/581. AA range:551-600
Molecular Weight80
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesPBS with 0.02% sodium azide and 50% glycerol pH 7.4.
Concentration1 mg/ml
DisclaimerFor research use only

Alternative Names

Anti-MYBL2 antibody, anti-BMYB antibody, anti-Myb-related protein B antibody, anti-B-Myb antibody, anti-Myb-like protein 2 antibody
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B-Myb (phospho Ser577) rabbit pAb

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using B-Myb (Phospho-Ser577/581) Antibody.

B-Myb (phospho Ser577) rabbit pAb

Immunofluorescence analysis of HeLa cells, using B-Myb (Phospho-Ser577/581) Antibody. The picture on the right is blocked with the phospho peptide.

B-Myb (phospho Ser577) rabbit pAb

Western blot analysis of lysates from K562 cells, using B-Myb (Phospho-Ser577/581) Antibody. The lane on the right is blocked with the phospho peptide.

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IF
Immunofluorescence
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

B-Myb (phospho Ser577) rabbit pAb (orb769177)

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50 μl
$ 250.00
100 μl
$ 380.00
DispatchUsually dispatched within 5-10 working days
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