ARRB1 Antibody (C-term)

SKU: orb1937801

This product can be custom made

Description

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Immunology & Inflammation, Neuroscience, Signal Transduction

Images & Validation

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Item 1 of 5

Tested Applications	FC, IF, IHC-P, WB
Dilution Range	WB - 1:1000, IF - 1:10-50, IHC-P - 1:10-50, FC - 1:10-50
Reactivity	Human
Predicted Reactivity	Mouse, Rabbit, Rat

Key Properties

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Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Clone No.	BTD53837
Immunogen	This ARRB1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 336-363 amino acids from the C-terminal region of human ARRB1. Antigen Region: 336-363 aa.
Target	ARRB1
Molecular Weight	47066 Da
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Form/Appearance	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Beta-arrestin-1, Arrestin beta-1, ARRB1, ARR1

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

All lanes: Anti-ARRB1 Antibody (C-term) at 1:1000 dilution + mouse liver lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 50 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

ARRB1 Antibody (C-term) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

ARRB1 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of ARRB1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

ARRB1 Antibody (C-term) western blot analysis in A549 cell line lysates (35 ug/lane). This demonstrates the ARRB1 antibody detected the ARRB1 protein (arrow).

Confocal immunofluorescent analysis of ARRB1 Antibody (C-term) with A549 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).