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Description
Research Area
Images & Validation
−| Tested Applications | ELISA, IF, WB |
|---|---|
| Dilution Range | WB: 1:500-2000 |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-322 of human Arginase 1 (ARG1) (NP_000036.2). |
| Molecular Weight | 40kDa |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | PBS with 0.05% proclin300,50% glycerol,pH7.3. |
| Concentration | 1 mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
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−Arginase I rabbit pAb Antibody [orb768903]
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Human, Mouse, Rat
Polyclonal
Unconjugated
50 μl, 100 μlArginase 1 Rabbit pAb, IRDye 800CW conjugated [orb2838174]
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Human
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IRDye800
100 μlArginase 1 Rabbit pAb, PerCP-Cy7 conjugated [orb2878310]
IF
Canine, Mouse, Porcine, Rabbit
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100 μl

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Immunofluorescence analysis of paraffin-embedded human liver cancer using Arginase 1 (ARG1) Rabbit pAb (orb167121) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Western blot analysis of various lysates using Arginase 1 (ARG1) Rabbit pAb (orb167121) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
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Protocol Information
Hou, Rui et al. Dietary Flavone Tectochrysin Exerts Anti-Inflammatory Action by Directly Inhibiting MEK1/2 in LPS-Primed Macrophages Mol Nutr Food Res, 62, (2018)
Arginase 1 (ARG1) Rabbit pAb (orb167121)
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