Vimentin Antibody

SKU: orb319707

Description

Our Anti-Vimentin primary antibody from Biorbyt is chicken polyclonal. It detects human, mouse, and rat Vimentin and is total IgY fraction.

Research Area

Cell Biology, Disease Biomarkers, Musculoskeletal & Connective Tissue Research

Images & Validation

• Tested Applications: ELISA, ICC, IHC, WB
• Dilution Range: WB: 0.736111111, IHC: 1:500-1:5000, ICC: 1:500-1:10,000
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Canine, Equine, Feline, Goat, Guinea pig, Hamster, Primate, Rabbit, Rodent, Sheep

Key Properties

• Antibody Type: Primary Antibody
• Host: Gallus
• Clonality: Polyclonal
• Isotype: IgY
• Immunogen: Recombinant human vimentin purified from E. coli.
• Target: Vimentin
• Molecular Weight: 50
• Purification: Total IgY fraction
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS + 10 mM NaN3.
• Concentration: 20 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CTRCT30 antibody, Epididymis luminal protein 113 antibody, FLJ36605 antibody, HEL113 antibody, VIM antibody, VIME_HUMAN antibody, Vimentin antibody

Immunoflourescence of HeLa cells labeled with anti-SAP49 (Cat. orb319660, red, 1:1000) and anti-vimentin (Cat. orb319707, green, 1:10,000). The blue is DAPI staining nuclear DNA.

Immunoflourescence of HeLa cells stained with Anti-Park7 (Cat. 1597-PARK, green, 1:1000) showing strong cytoplasmic staining and Anti-Vimentin (Cat. orb319707, red, 1:10,000) labeling cytoplasmic intermediate filaments. The blue is nuclear staining.

Immunoflourescence of mixed fibroblasts and PC12 cells labeled with anti-peripherin (Cat. orb319632, red, 1:500) and anti-vimentin (Cat. orb319707, green, 1:10,000). The blue is Hoechst staining nuclear DNA. Peripherin is labeled in only the PC12 cells cytoplasmic filaments and vimentin is only labeled in the fibroblasts intermediate filaments where peripherin is not present.

Immunofluorescence of HeLa cells showing strong granular nuclear staining of SAP49 (red, 1:1000) and vimentin (Cat. orb319707, green, 1:10,000). The blue is DAPI staining nuclear DNA.

Immunolabeling of HeLa cells stained with anti-nuclear pore complex antibody (Cat. orb319613, red, 1:100) and rabbit anti-vimentin antibody (Cat. orb319707, green, 1:10,000). The blue is DAPI staining nuclear DNA. The anti-nuclear pore complex antibody reveals strong granular labeling of the nuclei corresponding to the protein. The anti-vimentin antibody specifically labels intermediate filaments.

Immunostaining of cultured neonatal rat neurons and glia stained with anti-nestin antibody (Cat. orb319583, red, 1:500) and anti-vimentin antibody (Cat. orb319707, green, 1:500). The blue is Hoechst staining for nuclear DNA. Astrocytes and neuronal stem cells stain strongly and specifically in a clearly filamentous fashion with the anti-Nestin antibody. The presence of Nestin indicates that the cells are developing astrocytes, neuroblasts or undifferentiated neural stem cells.

Immunostaining of HeLa cells labelled with anti-vimentin antibody (Cat. orb319707, 1:10,000, green), anti-actin (red), and DAPI (blue) labelling of nuclear DNA. The vimentin antibody labels the intermediate filament network while the actin antibody labels the submembranous cytoskeleton, stress fibers, and bundles of actin associated with cell adhesion sites.

Immunostaining of HeLa cells stained with anti-nuclear pore complex antibody (Cat. orb319613, green, 1:100) and rabbit anti-vimentin antibody (Cat. orb319707, red, 1:500).

Immunostaining of mixed neuron/glial cultures stained with anti-Vimentin antibody (Cat. orb319707, 1:500, green) and anti-GFAP antibody (Cat. orb319549, 1:1000, red). Vimentin is expressed alone in fibroblastic and endothelial cells, which are the flattened cells in the middle of the image which appear green. Astrocytes may express primarily GFAP, or GFAP and vimentin, and so appear red (GFAP only) or golden yellow (GFAP and Vimentin).

Immunostaining of tumors and precursor PanIN lesions from 4 week old LSL-KrasG12D; Cdkn2alox/lox; p48Cre (KIC) mice that were treated with 2G8 and/or gemcitabine, Gem. (Anti-Vimentin, cat. orb319707, green; Anti-E-cadherin, red).

Western blot of NIH 3T3 cell lysate showing specific immunolabeling of the ~50 kDa Vimentin protein.

Quick Database Links

Gene Symbol

Vimentin

UniProt

P08670