VEGFA Antibody

• Catalog Number: orb1289717
• Category: Antibodies
• Description: The VEGFA Antibody is suitable for IF, WB. It is a Polyclonal, Unconjugated antibody which raised against Recombinant protein corresponding to human VEGFA. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Purification: The antibody was purified by immunogen affinity chromatography.
• Immunogen: Recombinant protein corresponding to human VEGFA. The exact sequence is proprietary.
• UniProt ID: Q00731, P15692, P16612
• Tested applications: IF, WB
• Dilution range: WB: 1:500-1:1000, IH: 1:50-1:200, IF:IC: 1:50-1:200
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: VEGF; Vascular endothelial growth factor A; VEGF-A
• Research Area: Cancer, Developmental Biology, Epigenetics
• Note: For research use only
• Entrez: 7422, 22339, 83785
• Expiration Date: 12 months from date of receipt.

Western blot analysis of VEGFA expression in HEK293T (A), A549 (B), mouse liver (C) whole cell lysates. (Predicted band size: 15-27; 34-45 kD; Observed band size: 16; 34; 45 kD)

Immunofluorescent analysis of VEGFA staining in HUVEC cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark.