UGT1A6 Rabbit Polyclonal Antibody

SKU: orb614141

Description

Anti-UGT1A6 Antibody. Tested in ELISA, IF, ICC, WB applications. This antibody reacts with Human.

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: ELISA, ICC, IF, WB
• Dilution Range: Western blot, 0.25-0.5μg/ml, Human Immunocytochemistry/Immunofluorescence, 2μg/ml, Human ELISA, 0.1-0.5μg/ml, -
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human UGT1A6 recombinant protein (Position: D27-S251).
• Target: UDP-glucuronosyltransferase 1A6
• Molecular Weight: 55-61 kDa
• Purification: Immunogen affinity purified.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Concentration: 500 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

UDPGT 1-6; UGT1*6; UGT1-06; UGT1.6; UGT-1F; UGT1F; UGT1A6; GNT1; UGT1; UDP-glucuronosyltransferase 1A6

IF analysis of UGT1A6 using anti-UGT1A6 antibody. UGT1A6 was detected in immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2 µg/mL rabbit anti-UGT1A6 Antibody overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

Western blot analysis of UGT1A6 using anti-UGT1A6 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50 ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-UGT1A6 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for UGT1A6 at approximately 55-61 KD. The expected band size for UGT1A6 is at 61 KD.

Quick Database Links

Gene Symbol

UDP-glucuronosyltransferase 1A6

UniProt

P19224