p57 Kip2/CDKN1C Rabbit Polyclonal Antibody

SKU: orb654296

Description

Anti-p57 Kip2/CDKN1C Antibody. Tested in WB applications. This antibody reacts with Human.

Research Area

Cardiovascular Research, Cell Biology, Disease Biomarkers, Hematology, Immunology & Inflammation, Stem Cell & Developmental Biology

Images & Validation

• Tested Applications: WB
• Dilution Range: Western blot, 0.1-0.25μg/ml, Human
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human p57 Kip2/CDKN1C, identical to the related mouse and rat sequences.
• Target: Cyclin-dependent kinase inhibitor 1C
• Molecular Weight: 57 kDa
• Purification: Immunogen affinity purified.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Concentration: 500 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

BWCR; BWS; CDKN1C; KIP2; p57; p57Kip2; WBS

Western blot analysis of p57 Kip2/CDKN1C using anti-p57 Kip2/CDKN1C antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human SW620 whole cell lysates, Lane 4: human Caco-2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-p57 Kip2/CDKN1C antigen affinity purified polyclonal antibody at 0.25 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for p57 Kip2/CDKN1C at approximately 57 KD. The expected band size for p57 Kip2/CDKN1C is at 57 KD.

Quick Database Links

Gene Symbol

Cyclin-dependent kinase inhibitor 1C

UniProt

P49918