MSH3 Antibody

• Catalog Number: orb256696
• Category: Antibodies
• Description: The MSH3 Antibody is suitable for IHC, WB. It is a Polyclonal, Unconjugated antibody which raised against KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human MSH3. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Purification: The antibody was purified by immunogen affinity chromatography.
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human MSH3. The exact sequence is proprietary.
• UniProt ID: P20585
• Tested applications: IHC, WB
• Dilution range: WB: 1:500-1000, IHC-P: 1:100-200
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: DUC1; DUG; DNA mismatch repair protein Msh3; hMSH3
• Research Area: Cancer, DNA Damage, Epigenetics
• Note: For research use only
• Entrez: 4437
• Expiration Date: 12 months from date of receipt.

Western blot analysis of MSH3 expression in A549 (A), MCF7 (B) whole cell lysates. (Predicted band size: 127 kD; Observed band size: 127 kD)

Immunohistochemical analysis of MSH3 staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.