CD47 Biosimilar Antibody

• Catalog Number: orb1173629
• Category: Antibodies
• Description: Anti-CD47(lemzoparlimab biosimilar) mAb
• Target: CD47
• Clonality: Monoclonal
• Species/Host: Human Humanized
• Isotype: IgG4
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Powder
• Purification: Purified from cell culture supernatant by affinity chromatography
• UniProt ID: Q08722
• Tested applications: ELISA, FC
• Dilution range: ELISA 1:5000-10000 Flow Cyt 1:100
• Application notes: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.
• Antibody Type: Biosimilar Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: CD47MER6IAP
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

ELISA plate pre-coated by 1 μg/mL (100 μL/well) Human CD47 protein, mFc-His Tag orb689369 can bind Anti-CD47 Neutralizing antibody (orb1173629) in a linear range of 3.2-400 ng/ml.

Jurkat cell line were surface stained with anti-CD47 (orb1173629) (Blue histogram) and isotype control (Red histogram) 1 μg/ml followed by Alexa 488-conjugated Goat anti-Human IgG secondary antibody.

Flow cytometry analysis of antigen binding of anti-human CD47 mAb (orb1173629). (A) orb1173629 does not bind to CHO-S cells that do not express CD47. (B) A clear peak shift of orb1173629 was seen compared to the control when incubated with CD47-expressing 8226 cells, indicating strong binding of orb1173629 to CD47. Antibodies were incubated at 5 μg/mL.