CD20/MS4A1 Antibody

• Catalog Number: orb182382
• Category: Antibodies
• Description: CD20/MS4A1 Antibody
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Lyophilized
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Purification: Immunogen affinity purified.
• Immunogen: E.coli-derived human CD20 recombinant protein (Position: M1-D261). Human CD20 shares 75% amino acid (aa) sequence identity with mouse CD20.
• UniProt ID: P11836
• MW: 35 kDa
• Tested applications: FC, ICC, IF, IHC, IHC-Fr, WB
• Application notes: Western blot, 0.1-0.5μg/ml Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml Immunohistochemistry (Frozen Section), 0.5-1μg/ml Immunocytochemistry, 0.5-1μg/ml Immunofluorescence, 2μg/ml, Human Flow Cytometry (Fixed), 1-3μg/1x106cells. Add 0.2ml of distilled water will yield a concentration of 500ug/ml
• Cross Reactivity: No cross-reactivity with other proteins
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: B-lymphocyte antigen CD20; B-lymphocyte surface an
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of U937 cells using anti-CD20 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

Flow Cytometry analysis of A431 cells using anti-CD20 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of CD20 using anti-CD20 antibody.Lane 1:human Daudi cell.

IF analysis of CD20 using anti-CD20 antibody.CD20 was detected in paraffin-embedded section of human B lymphocytic tumor tissues.

IHC analysis of CD20 using anti-CD20 antibody.CD20 was detected in paraffin-embedded section of Human Tonsil Tissue.

IHC analysis of CD20 using anti-CD20 antibody.CD20 was detected in paraffin-embedded section of Human Tonsil Tissue.