CD133 Antibody

SKU: orb1821778

Description

Our CD133 rabbit polyclonal primary antibody from Biorbyt is produced in-house. It detects human CD133 and is antigen affinity purified.

Research Area

Disease Biomarkers, Immunology & Inflammation; Cell Biology, Stem Cell & Developmental Biology

Images & Validation

• Tested Applications: IHC, WB
• Dilution Range: WB: 1:500-1:1000, IHC: 1:100-1:250
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Synthetic peptide corresponding to amino acids from an internal sequence of the human Prominin-1 (CD133) protein.
• Target: CD133
• Purification: Antigen Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS + 0.02% NaN3
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

PROM1, Antigen AC133, Prominin-like protein 1, PROML1, MSTP061

Chromatin Immunoprecipitation showing staining of CD133 in Bone Marrow Mononuclear Cells by immunofluorescence.

Immunohistochemistry of human hepatocarcinoma (HC) tissue showing specific staining of CD133.

Immunostaining of normal ovarian and tumor tissue sections showing specific labeling of CD133. Staining of CD133 in OSE layer (A, B) as well as cortex (C) reveals specific CD133+ cells with relatively higher cell numbers in BL and HG. Area within dotted lines in BN OSE (A) are magnified in (B) while elliptical/spindle shaped CD133+ cells in cortex from various fields were represented in the composite image in (C) of BN and HG. Large CD133+ cells in cortex were also observed.

Immunostaining of normal ovarian and tumor tissue sections showing specific labeling of CD133. Staining of CD133 in OSE layer (A, B) as well as cortex (C) reveals specific CD133+ cells with relatively higher cell numbers in BL and HG. Area within dotted lines in BN OSE (A) are magnified in (B) while elliptical/spindle shaped CD133+ cells in cortex from various fields were represented in the composite image in (C) of BN and HG. Large CD133+ cells in cortex were also observed.

Western Blot of Y79 cells showing specific immunolabeling of human CD133. Band is at ~120 kDa due to protein glycosylation.

Quick Database Links

Gene Symbol

CD133

UniProt

O43490