Anti-B7-H3 (enoblituzumab biosimilar) mAb

• Catalog Number: orb689315
• Category: Antibodies
• Description: Anti-B7-H3 (enoblituzumab biosimilar) mAb
• Target: B7-H3
• Clonality: Monoclonal
• Species/Host: Human Humanized
• Isotype: IgG1
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Powder
• Purification: Purified from cell culture supernatant by affinity chromatography
• UniProt ID: Q5ZPR3
• Tested applications: ELISA, FC
• Dilution range: ELISA 1:5000-10000; Flow Cyt 1:100
• Application notes: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.
• Antibody Type: Biosimilar Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: B7-H3;CD276;B7 homolog 3
• Note: For research use only

ELISA plate pre-coated by 2 μg/ml (100 μL/well) Human B7-H3, mFc-His tagged protein orb689373 can bind Anti-B7-H3 Neutralizing antibody in a linear range of 0.24-31.25 ng/ml.

Flow cytometry analysis with 1 μg/mL Anti-B7-H3 (enoblituzumab biosimilar) mAb (orb689315) on Hela cells (Blue histogram) or isotype control mAb on Hela cells (Red histogram).

Flow cytometry analysis of antigen binding of anti-human B7-H3 mAb (orb689315). (A) A clear peak shift of orb689315 was seen compared to the control when incubated with B7-H3-expressing Hela cells, indicating strong binding of orb689315 to B7-H3. Antibodies were incubated at 2 μg/mL.