Anti-ACTN1/2/3/4 Antibody

• Catalog Number: orb224153
• Category: Antibodies
• Description: Rabbit polyclonal antibody to ACTN1/2/3/4
• Target: ACTN1; ACTN2; ACTN3; ACTN4
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat, Zebrafish
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Purification: The antibody was purified by immunogen affinity chromatography.
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human ACTN1/2/3/4. The exact sequence is proprietary.
• UniProt ID: P12814, Q9Z1P2, P35609, O43707, Q9QXQ0, P57780, Q9JI91, O88990, Q08043, Q7TPR4
• Tested applications: IH, WB
• Dilution range: WB: 1:500-1:1000, IHC-P: 1:100-1:200, IP: 1:10-1:100
• Antibody Type: Primary Antibody
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti ACTN1 antibody, anti F-actin cross-linking pr
• Note: For research use only
• Entrez: 88, 11472, 63836, 81, 109711, 11474, 87, 60595, 89, 81634

Western blot analysis of ACTN1/2/3/4 expression in A375 (A), U2OS (B), DLD (C), mouse heart (D), mouse muscle (E), rat heart (F), rat muscle (G) whole cell lysates. (Predicted band size: 103; 104 kD; Observed band size: 105 kD)

Immunohistochemical analysis of ACTN1/2/3/4 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.