Angiotensin II Type 1 Receptor Rabbit Polyclonal Antibody

SKU: orb4245

Description

Angiotensin II Type 1 Receptor Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Sheep

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human AT1R (151-250/359aa)
• Target: AGTR1
• Molecular Weight: 39 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

AG2S; Agtr 1; Agtr1; Agtr1a; AGTR1B; Angiotensin II receptor type 1; Angiotensin II type 1 receptor; AT-1B; AT-1r; AT1; At1a; AT1AR; AT1B; AT1BR; AT2R1; AT2R1A; AT2R1B; HAT1R; Type 1 angiotensin II receptor; AGTR1_HUMAN; Type-1 angiotensin II receptor; Angiotensin II type-1 receptor; AT1R; Ang II; Angiotensin II receptor type 1; Angiotensin II type 1 receptor; Angiotensin receptor 1; Angiotensin receptor 1B; AT 1B; AT1; AT1R.

Paraformaldehyde-fixed, paraffin embedded (mouse kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Angiotensin II Type 1 Receptor) Polyclonal Antibody, Unconjugated (orb4245) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Angiotensin II Type 1 Receptor) Polyclonal Antibody, Unconjugated (orb4245) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: HepG2 (human) cell Lysate at 40 ug, Primary: Anti-Angiotensin II Type 1 Receptor (orb4245) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 39kD, Observed band size: 49kD.

Sample: Liver (mouse) Lysate at 40 ug, Primary: Anti-Angiotensin II Type 1 Receptor (orb4245) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 39kD, Observed band size: 36kD.

Tissue/Cell: human pneumonia tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-AT1R/AGTR1 Polyclonal Antibody, Unconjugated (orb4245) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

AGTR1

UniProt

P30556