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| Catalog Number | orb556835 |
|---|---|
| Category | Antibodies |
| Description | Rabbit polyclonal antibody to alpha Actinin 4 |
| Target | alpha Actinin 4 |
| Clonality | Polyclonal |
| Species/Host | Rabbit |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Form/Appearance | Liquid: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
| Concentration | 1.03 mg/ml |
| Buffer/Preservatives | 0.01% Thimerosal. 0.1M Tris-Glycine (pH7), 10% Glycerol |
| Purity | Purified by antigen-affinity chromatography. |
| Purification | Purified by antigen-affinity chromatography. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human alpha Actinin 4. The exact sequence is proprietary. |
| UniProt ID | O43707 |
| MW | 105 |
| Tested applications | ICC, IHC-P, WB |
| Dilution range | WB: 1:500-1:3000 , IF: 1:100-1:1000 , IHC-P: 1:100-1:1000 |
| Application notes | Positive control: Mouse brain , PC-12 , A431 , HepG2 , NIH-3T3 |
| Antibody Type | Primary Antibody |
| Storage | Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°lC or below. Avoid multiple freeze-thaw cycles. |
| Alternative names | actinin alpha 4 , ACTININ-4 , FSGS , FSGS1 |
| Research Area | Epigenetics |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
![alpha Actinin 4 antibody [N2C1], Internal](/images/pub/media/catalog/product/a/l/alpha-actinin-4-antibody_orb556835_ifa_1.jpg)
Immunofluorescence analysis of HeLa cells using alpha Actinin 4 antibody
![alpha Actinin 4 antibody [N2C1], Internal](/images/pub/media/catalog/product/a/l/alpha-actinin-4-antibody_orb556835_ihc_1.jpg)
Immunohistochemical staining of A549 xenograft using alpha Actinin 4 antibody
![alpha Actinin 4 antibody [N2C1], Internal](/images/pub/media/catalog/product/a/l/alpha-actinin-4-antibody_orb556835_wb_1.jpg)
Western blot analysis of A431(Lane 1), HepG2(Lane 2) using alpha Actinin 4 antibody
![alpha Actinin 4 antibody [N2C1], Internal](/images/pub/media/catalog/product/a/l/alpha-actinin-4-antibody_orb556835_wb_2.jpg)
Western blot analysis of A431(Lane 1), HepG2(Lane 2) using alpha Actinin 4 antibody
![alpha Actinin 4 antibody [N2C1], Internal](/images/pub/media/catalog/product/a/l/alpha-actinin-4-antibody_orb556835_wb_3.jpg)
Western blot analysis of A431(Lane 1), HepG2(Lane 2) using alpha Actinin 4 antibody
![alpha Actinin 4 antibody [N2C1], Internal](/images/pub/media/catalog/product/a/l/alpha-actinin-4-antibody_orb556835_wb_4.jpg)
Western blot analysis of A431(Lane 1), HepG2(Lane 2) using alpha Actinin 4 antibody
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