Akt1+2+3/Pan-AKT Antibody

• Catalog Number: orb1294323
• Category: Antibodies
• Description: Rabbit polyclonal antibody to AKT (pan)
• Target: Akt1+2+3 / Pan-AKT
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit-IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Immunogen: Synthetic peptide / encompassing a sequence within the C-terminus region.
• Tested applications: IF, WB
• Dilution range: Western Blot 1:1000-1:2000 Immunofluorescence 1:200-1:500
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Note: For research use only

AKT (pan) antibody at 1/1000 dilution, Lysates at 60 µg per lane. This blot was produced using a 10% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294323 overnight at 4°C.

AKT (pan) antibody at 1/1000 dilution, Lysates at 60 µg per lane. This blot was produced using a 10% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294323 overnight at 4°C.

Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294323 anti-AKT (pan) antibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.

Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294323 anti-AKT (pan) antibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.