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Akt (Phospho-Ser473) Antibody

Catalog Number: orb127667

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SizePriceQuantity
25 μg$ 110.00
50 μg$ 170.00
100 μg$ 260.00
200 μg$ 380.00
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DispatchUsually dispatched within 5-10 working days
Product Properties
Catalog Numberorb127667
CategoryAntibodies
DescriptionAkt (Phospho-Ser473) Antibody is an unconjugated polyclonal antibody. It is suitable for use in IF, IHC, WB. It exhibits reactivity with Human, Mouse, Rat.
ClonalityPolyclonal
Species/HostRabbit
ConjugationUnconjugated
ReactivityHuman, Mouse, Rat
Concentration1mg/ml
ImmunogenC-terminal
UniProt IDP31749
Tested applicationsIF, IHC, WB
Dilution rangeWB: 1:500-1:3000, ELISA: 1:1000, IHC-P: 1:50-1:200
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Alternative namesanti AKT 1 antibody, anti AKT antibody, anti AKT1
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Research AreaApoptotic, Cancer, Developmental Biology, Epigenet
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NoteFor research use only
Expiration Date12 months from date of receipt.
Images
Akt (Phospho-Ser473) Antibody

Western blot analysis of extracts from K562 using Akt (phospho-Ser473) antibody

Akt (Phospho-Ser473) Antibody

Western blot analysis of extracts from JK cells using Akt (phospho-Ser473) antibody

Akt (Phospho-Ser473) Antibody

Immunohistochemistry analysis of formalin-fixed and paraffin-embedded human breast carcinoma tissue using Akt (phospho-Ser473) antibody

Akt (Phospho-Ser473) Antibody

Confocal immunofluorescence analysis of A549 cells using Akt (phospho-Ser473) antibody

Akt (Phospho-Ser473) Antibody

Western blot analysis of extracts from K562 cells using CIITA antibody (see review by T. Sharma)

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Reviews

Akt (Phospho-Ser473) Antibody (orb127667)

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Applications: IP, IF, WB| Reactivity: Human

For western, I used 1:1000 dilution and prepared the antibody in 3% BSA. It worked efficiently. A single band at the expected size was obtained. For IF, I used 1:50 dilution in % BSA solution prepared in 1X PBS. For IP, I used 2ug for 500 ug of whole cell lysate of the MCF-7 cell line. It's a very good antibody and provides reproducible results.

- Tanisha SharmaNCCS