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| Catalog Number | orb767259 |
|---|---|
| Category | Antibodies |
| Description | Rabbit polyclonal antibody to Acetyl Lys proteins |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Reactivity | Human, Monkey, Mouse, Plant, Rat |
| Concentration | 1 mg/ml |
| Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Immunogen | Synthesized acetyl-peptide derived from human acetylation Lys proteins. |
| MW | 20,40,80,175 |
| Tested applications | ELISA, IF, IHC-P, WB |
| Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications. |
| Source | Rabbit |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Note | For research use only |
Immunofluorescence analysis of HeLa cells, using Lys-Acetylated Proteins Antibody. The picture on the right is blocked with the synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using Lys-Acetylated Proteins Antibody. The picture on the right is blocked with the synthesized peptide.
Western blot analysis of lysates from COS7 cells treated with TSA 400uM 24h, using Lys-Acetylated Proteins Antibody. The lane on the right is blocked with the synthesized peptide.
Western blot analysis of lysates from 1) Hela, 2) mouse brain, (Green) primary antibody was diluted at 1:1000, 4°C over night, secondary antibody was diluted at 1:10000, 37°C 1hour.
