A-Raf proto-oncogene, serine/threonine kinase Antibody

SKU: orb555953

Description

Rabbit polyclonal antibody to A-RAF

Research Area

Cancer, Epigenetics

Images & Validation

• Tested Applications: ICC, IHC-P, WB
• Dilution Range: WB: 1:500-1:3000 , IF: 1:100-1:1000 , IHC-P: 1:100-1:1000
• Reactivity: Human, Mouse
• Application Notes:
  Positive control: HeLa , NIH-3T3

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human A-RAF. The exact sequence is proprietary.
• Target: A-RAF
• Molecular Weight: 68
• Purity: Purified by antigen-affinity chromatography.
• Purification: Purified by antigen-affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°lC or below. Avoid multiple freeze-thaw cycles.
• Form/Appearance: Liquid: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Buffer/Preservatives: 0.1M Tris, 0.1M Glycine, 10% Glycerol, 0.01% Thimerosal.
• Concentration: 0.85 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

A-Raf proto-oncogene, serine/threonine kinase , A-RAF , ARAF1 , PKS2 , RAFA1

Immunofluorescence analysis of A431 cells using A-RAF antibody

Immunohistochemical staining of Cal27 Xenograft using A-RAF antibody

Western blot analysis of NIH-3T3 cells using A-RAF antibody

Western blot analysis of HeLa cells using A-RAF antibody

Quick Database Links

Gene Symbol

A-RAF

UniProt

P10398