5-Vinyl-uridine (5-VU)

• Catalog Number: orb1734970
• Category: Small Molecules
• Description: 5-VU (5-Vinyl-uridine) can potentially be used as a replacement for BrU (5-Bromo-uridine) or the copper-catalyst requiring 5-EU (5-Ethynyl-uridine) to measure de novo RNA synthesis in proliferating cells. 5-VU is cell permeable and might incorporate into nascent RNA instead of its natural analog uridine. This assumption is based on the previously demonstrated suitability of 5-VdU (5-Vinyl-2'-deoxyuridine) for DNA synthesis monitoring. The resulting vinyl-functionalized RNA could subsequently be detected via Cu(I)-free Alkene-Tetrazine Ligation that offers the choice to introduce a Biotin group (via Tetrazines of Biotin) for subsequent purification tasks or a fluorescent group (via Tetrazines of fluorescent dyes) for subsequent microscopic imaging.
• Form/Appearance: solid; Color: white to off-white
• Purity: ≥ 98% (HPLC)
• MW: Theoretical MW: 270.24 g/mol; Detected MW: 270.09 g/mol
• Application notes: RNA synthesis monitoring (potentially). ; Spectroscopic Propertie: λmax 288 nm, ε 8.5 L mmol-1 cm-1, (Methanol).
• Solubility (25°C): DMSO, methanol
• CAS Number: 55520-64-4
• Formula: C₁₁H₁₄N₂O₆
• SMILES: n1(cc(c(=O)[nH]c1=O)C=C)[C@H]1[C@@H]([C@@H]([C@H](O1)CO)O)O
• Storage: store at -20 °C. Short term exposure (up to 1 week cumulative) to ambient temperature possible
• Note: For research use only
• Expiration Date: 12 months from date of receipt.