I2959

SKU: orb2944117

Description

I2959, widely known as Irgacure 2959, is a photoinitiator extensively used for ultraviolet light-induced photocrosslinking of hydrogels in biomedical applications and is particularly utilized in combination with carboxymethylcellulose (CMA) hydrogels as a bioink for bio 3D printing applications; furthermore, I2959 is recognized for its high radical generation efficiency under UV exposure but also exhibits concentration-dependent cytotoxicity to rapidly dividing cell lines.

Key Properties

• CAS Number: 106797-53-9
• MW: 224.25
• Purity: 97.97%
• Formula: C₁₂H₁₆O₄
• SMILES: O=C(C1=CC=C(OCCO)C=C1)C(O)(C)C
• Target: Others
• Solubility: DMSO:80 mg/mL (356.74 mM)

Bioactivity

• In Vitro:
  Encapsulation of Saos-2 cells in photochemically cross-linked CMA hydrogels: 1. Proliferate the human osteosarcoma cell line Saos-2 in RPMI medium (containing 15% FBS, 1% L-glutamine, and 1% penicillin/streptomycin). 2. Resuspend the cells in a neutralized CMA solution supplemented with 0.02% or 0.1% photoinitiator I2959. 3. Add 45 µL of the cell suspension (approximately 5,000 cells) to each well of a 96-well plate and incubate at 37°C for 30 minutes to allow for initial gelation. 4. Irradiate the hydrogels with 365 nm UV light for either 1 minute or 10 minutes to complete photocrosslinking; a control hydrogel without UV exposure was also prepared. 5. Culture the encapsulated cell hydrogels in α-MEM medium (supplemented with 50 µg/mL ascorbic acid, 10 mM β-glycerophosphate, 10% FBS, and 1% penicillin/streptomycin) for up to 14 days.

Storage & Handling

• Storage: keep away from direct sunlight,keep away from moisture | Powder: -20°C for 3 years | In solvent: -80°C for 1 year
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Compound Identifiers

PubChem CID

86266