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Catalog Number | orb766752 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody to Endothelin 1 |
Clonality | Polyclonal |
Isotype | IgG |
Conjugation | Unconjugated |
Reactivity | Human, Mouse, Rat |
Concentration | 1 mg/ml |
Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Immunogen | The antiserum was produced against synthesized peptide derived from the Internal region of human EDN1. AA range:131-180 |
MW | 25 |
Tested applications | ELISA, IHC-P, WB |
Dilution range | IHC-p: 100-300.Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications. |
Source | Rabbit |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Alternative names | Anti-EDN1 antibody, anti-Endothelin-1 antibody, an Read more... |
Note | For research use only |
Western Blot analysis of mouse lung cells using ET-1 Polyclonal Antibody. Secondary antibody was diluted at 1:20000.
Western blot analysis of lysate from mouse lung, using EDN1 Antibody.
Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
FC, IF | |
Bovine, Canine, Equine, Guinea pig, Porcine, Sheep | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Pacific Blue |
FC, IF | |
Bovine, Canine, Equine, Guinea pig, Porcine, Sheep | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
BF700 |
IF | |
Bovine, Canine, Equine, Porcine, Rabbit | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
PerCP/Cy7 |
IF | |
Bovine, Canine, Equine, Porcine, Rabbit | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Pacific Blue |