Cdc25B (phospho Ser323) rabbit pAb

SKU: orb764158

Description

Rabbit polyclonal antibody to Cdc25B (phospho-Ser323)

Images & Validation

• Tested Applications: ELISA, IHC-P, WB
• Dilution range: Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
• Reactivity: Human, Mouse, Rat

Key Properties

• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human CDC25B around the phosphorylation site of Ser323. AA range:289-338
• Molecular Weight: 70
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

Anti-CDC25B antibody, anti-CDC25HU2 antibody, anti-M-phase inducer phosphatase 2 antibody, anti-Dual specificity phosphatase Cdc25B antibody

Western Blot analysis of various cells using Phospho-Cdc25B (S323) Polyclonal Antibody diluted at 1:1000.

Western Blot analysis of VEC cells using Phospho-Cdc25B (S323) Polyclonal Antibody diluted at 1:1000.

Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using CDC25B (Phospho-Ser323) Antibody.