CD74 Antibody: HRP

• Catalog Number: orb396365
• Category: Antibodies
• Description: Mouse monoclonal antibody against CD74 conjugated to HRP.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 3D7
• Tested applications: ELISA, IHC, WB
• Reactivity: Human
• Isotype: IgG1
• Immunogen: Human CD74 invariant chain synthetic peptide.
• Concentration: 1 mg/ml
• Dilution range: WB (1:1000); FACS (1:50)
• Conjugation: HRP
• MW: 33 kDa
• Target: CD74
• Entrez: 972
• UniProt ID: P04233
• NCBI: NP_001020329.1
• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 73.64mM Carbonate, 54.55mM Ethanolamine, 45.45mM Cyanoborohydride, 18.18mM Sodium Hydroxide and 0.23mM Citrate in dH2O
• Alternative names: DHLAG antibody, HLA DR gamma antibody, HLADG antib
• Note: For research use only
• Application notes: A 1:1000 dilution of SMC-268 was sufficient for detection of CD74 in 15 µg of B-Cell Lymphoma cell line Raji by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis using Mouse Anti-CD74 Monoclonal Antibody, Clone 3D7. Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 90% Methanol. Primary Antibody: Mouse Anti-CD74 Monoclonal Antibody at 1:100 for 30 min on ice. Secondary Antibody: Goat Anti-Mouse: PE at 1:100 for 20 min at RT. Isotype Control: Non Specific IgG. Cells were subject to heat shock at 42°C for 2 hours and transferred to CO2 incubator at 37°C for 1 hour before trypsinization.

Western Blot analysis of Human Lymphoblastoid cell line (Raji) showing detection of 33-35 kDa CD74 protein using Mouse Anti-CD74 Monoclonal Antibody, Clone 3D7. Lane 1: Molecular Weight Ladder (MW). Lane 2: Raji cell lysate. Load: 15 μg. Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-CD74 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 33-35 kDa.