CD45RA Antibody (Leukocyte marker)

• Catalog Number: orb385690
• Category: Antibodies
• Description: CD45RA is an isoform of the human leukocyte common antigen (CD45). Human CD45 contains three exons which encode peptide segments designated A, B and C, respectively. The differential splicing of the exons generates at least five isoforms, ABC, AB, BC, B and O. This antibody reacts with ABC and BC isoforms. CD45RA is expressed on 40-50% of peripheral CD4+ T-cells, 50% of peripheral CD8+ T-cells, B-cells, and leukemic B-cell lines. T-cells expressing CD45RA are naive or virgin T-cells. T-cells expressing CD45RO are memory T-cells. CD45RA and CD45RO define complementary, predominantly non-overlapping populations of resting peripheral T-cells. This MAb is useful in study on the subpopulation of CD4+ or CD8+ T-cells. It can especially be used to differentiate T-cell lymphomas (CD45RO +ve) from B cell lymphomas (CD45RA +ve).
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgG2a, kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Immunogen: Stimulated human leukocytes were used as immunogen for this CD45RA antibody.
• UniProt ID: P08575
• Tested applications: FACS, IF, IHC-P, WB
• Dilution range: Flow cytometry: 0.5-1ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT
• Application notes: Due to variation in protocol and secondary antibody used, the CD45RA antibody may need to be titered for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Antibody Type: Primary Antibody
• Clone Number: PTPRC/818
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Research Area: Epigenetics, Infectious Diseases
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

IHC test of FFPE human tonsil probed with CD45RA antibody. HIER: boil tissue sections in 10mM citrate buffer, pH6, for 10-20 min followed by cooling at RT for 20 minutes.

Flow cytometry testing of PFA-fixed human Jurkat cells with CD45RA antibody (clone PTPRC/818); Red = isotype control, Blue = CD45RA antibody.

Immunofluorescence staining of PFA-fixed human Jurkat cells with CD45RA antibody (clone PTPRC/818, green) and Reddot nuclear stain (red).

Western blot testing of human spleen lysate with CD45RA antibody (clone PTPRC/818). Expected molecular weight: 147-220 kDa depending on glycosylation level.